What is the basic principle behind Veterinary ELISA Test Kits?

Veterinary ELISA Test Kits operate on the principle of antigen-antibody interaction. The test begins by immobilizing specific antigens from the sample onto a solid surface, typically a microplate well. Then, the sample is added, allowing any antibodies present to bind to the immobilized antigens. After washing away unbound substances, an enzyme-linked secondary antibody is introduced, binding specifically to the primary antibodies. This enzyme-linked complex generates a detectable signal when a substrate is added. The intensity of the signal is proportional to the amount of target antibody present in the sample, enabling disease diagnosis.